Description
Cultrex®BasementMembraneExtract(BME)isasolubleformofbasementmembranepurifiedfromEngelbreth-Holm-Swarm(EHS)tumor.Thisextractprovidesanaturalextracellularmatrixhydrogelthatpolymerizesat37°Ctoformareconstitutedbasementmembrane.Basementmembranesarecontinuoussheetsofspecializedextracellularmatrixthatformaninterfacebetweenendothelial,epithelial,muscle,orneuronalcellsandtheiradjacentstromaandthatplayanessentialroleintissueorganizationbyinfluencingcelladhesion,migration,proliferation,anddifferentiation.ThemajorcomponentsofBMEincludelaminin,collagenIV,entactin,andheparansulfateproteoglycans.
Cultrex®BMEType3isphysiologicallyalignedwiththeinvivoenvironmentandisrecommendedforxenografts,tumorgrafts,andotherinvivoapplications.
Qualitycontrolspecifications:
- Mousecoloniesareroutinelyscreenedforpathogensusing mouseantibodyproduction(MAP)testing.
- Engelbreth-Holm-Swarm(EHS)tumorisLDEV-free.
- PathClear®–testednegativebyPCRfor 17bacterialandvirusstrainstypicallyincludedinmouseantibodyproduction(MAP)testing,plus13additionalmurineinfectiousagentsincludingLDEV,foratotalof31organismsandviruses.
- Nobacterialorfungalgrowthdetectedafterincubationat37°Cfor14daysfollowingUSPsterilitytestingguidelines.
- Endotoxinconcentration≤8EU/mlbyLimulusAmoebocyteLysate(LAL)assay.
- Proteinconcentrationof12–18mg/ml.
- NomycoplasmacontaminationdetectedbyPCR.
- ProvidedinRoswellParkMemorialInstituted(RPMI) mediumwith10µg/mlgentamycin.
- GelstABIlitytestedforaperiodof14daysat37°C.
- BIOLOGicalactivitydeterminedforeachlot:
- TumorGrowthAssay–BMEType3supportsproliferationandgrowthofbreastcancercells(MCF7)embeddedinthematrixforminimumof8days.
| Catalog#3632-010-02includes: | ||
| CatalogNumber | Description | Qty |
| 3632-005-02 | Cultrex®BasementMembraneExtract,Type3,PathClear® | 2 |
GeneticIdentificationofSEMA3FasanAntilymphangiogenicMetastasisSuppressorGeneinHeadandNeckSquamousCarcinoma
ColleenL.Doçi,ConstantinosM.Mikelis,MichailS.Lionakis,AlfredoA.Molinolo,andJ.SilvioGutkind
CancerRes.,Jul2015;75:2937–2948.
http://cancerres.aacrjournals.org/cgi/content/abstract/75/14/2937
