Description

Cultrex®BasementMembraneExtract(BME)isasolubleformofbasementmembranepurifiedfromEngelbreth-Holm-Swarm(EHS)tumor.Thisextractprovidesanaturalextracellularmatrixhydrogelthatpolymerizesat37°Ctoformareconstitutedbasementmembrane.Basementmembranesarecontinuoussheetsofspecializedextracellularmatrixthatformaninterfacebetweenendothelial,epithelial,muscle,orneuronalcellsandtheiradjacentstromaandthatplayanessentialroleintissueorganizationbyinfluencingcelladhesion,migration,proliferation,anddifferentiation.ThemajorcomponentsofBMEincludelaminin,collagenIV,entactin,andheparinsulfateproteoglycans.

Cultrex®BMEisaBasementMembraneMatrix thathasbeendeveloped,producedandqualifiedforgeneralcellcultureapplications.

Qualitycontrolspecifications:

• Mousecoloniesareroutinelyscreenedforpathogensusing mouseantibodyproduction(MAP)testing.
• Engelbreth-Holm-Swarm(EHS)tumorisLDEV-free.
• PathClear®–testednegativebyPCRfor17bacterialandvirusstrainstypicallyincludedinmouseantibodyproduction(MAP)testing,plus13additionalmurineinfectiousagentsincludingLDEV,foratotalof31organismsandviruses.
• Nobacterialorfungalgrowthdetectedafterincubationat37°Cfor14daysfollowingUSPsterilitytestingguidelines.
• Endotoxinconcentration≤8EU/mlbyLimulusAmoebocyteLysate(LAL)assay.
• Proteinconcentrationof12–18mg/ml.
• NomycoplasmacontaminationdetectedbyPCR.
• ProvidedinDulbecco’sModifiedEagle’smedium(DMEM)withoutphenolred,with10µg/mlgentamicinsulfate.
• GelstABIlitytestedforaperiodof14daysat37°C.
• BIOLOGicalactivitydeterminedforeachlot:
  • Tubeformationassay–BMEpromotesformationofcapillary-likestructuresbyhuman(HBMVEC;HUVEC)ormouse(SVEC4-10)endothelialcells.

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Chhetri,NoraBengoa-Vergniory,ChristophZenzmaier,PeterBerger,JonathanWaxman,andRobertKypta”
Carcinogenesis,Jan2016;37:18–29
http://carcin.oxfordjournals.org/cgi/content/abstract/37/1/18

Real-timemonitoringofNKCC2endocytosisbytotalinternalreflectionfluorescence(TIRF)microscopy
AnkitaBachhawatJaykumar,PauloS.Caceres,IbrahimSablaban,BakhosA.Tannous,andPabloA.Ortiz
AmJPhysiolRenalPhysiol,Jan2016;310:F183–F191
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Cabozantinib(XL184)InhibitsGrowthandInvasionofPreclinicalTNBCModels
“MansourehSameni,ElizabethA.Tovar,CurtJ.Essenburg,AnitaChalasani,ErikS.Linklater,Andrew
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Clin.CancerRes.,Feb2016;22:923–934”
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3-DimensionalPatient-DerivedLungCancerAssaysRevealResistancetoStandards-of-CarePromotedbyStromalCellsbutSensitivitytoHistoneDeacetylaseInhibitors
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SuppressionofER{beta}signalingviaER{beta}knockoutorantagoNISTprotectsagainstbladdercancerdevelopment
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Theinterplaybetweenhypoxia,endothelialandmelanomacellsregulatesvascularizationandcellmotilitythroughendothelin-1andvascularendothelialgrowthfactor,
FrancescaSpinella,ValentinaCaprara,RobertaCianfrocca,LauraRosanò,ValerianaDiCastro,EmirenaGarrafa,PierGiorgioNatali,andAnnaBagnato
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InhibitionofTumorGrowthandAngiogenesisbySP-2,anAnti-Lectin,Galactoside-BindingSoluble3BindingProteinLGALS3BPAntibody
SaraTraini,EnzaPiccolo,NicolaTinari,CosmoRossi,RossanaLaSorda,FrancescaSpinella,AnnaBagnato,RossanoLattanzio,MauriziaD’Egidio,AnnalisaDiRisio,FedericaTomao,AntoninoGrassadonia,MauroPiantelli,ClaraNatoli,andStefanoIacobelli
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SimianImmunodeficiencyVirusandHumanImmunodeficiencyVirusType1MatrixProteinsSpecifyDifferentCapabilitiesToModulateBCellGrowth
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TheNuclearReceptorPeroxisomeProliferator-activatedReceptor-β/(PPARβ/)PromotesOncogene-inducedCellularSenescencethroughRepressionofEndoplasmicReticulumStress
BokaiZhu,ChristinaH.Ferry,LaurenK.Markell,NicholasBlazanin,AdamB.Glick,FrankJ.Gonzalez,andJeffreyM.Peters
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PI3K/mTORDualInhibitorVS-5584PreferentiallyTargetsCancerStemCells
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TheMEF2–HDACaxiscontrolsproliferationofmammaryepithelialcellsandaciniformationinvitro
AndreaClocchiatti,ErosDiGiorgio,GiuliaViviani,CharlesStreuli,AndreaSgorbissa,RaffaellaPicco,ValentinaCutano,andClaudioBrancolini
J.CellSci.,Nov2015;128:3961–3976.
http://jcs.biologists.org/cgi/content/abstract/128/21/3961

Dickkopf-3regulatesprostateepithelialcellacinarmorphogenesisandprostatecancercellinvasionbylimitingTGF-β-dependentactivationofmatrixmetalloproteases
DianaRomero,ZainabAl-Shareef,IrantzuGorroño-Etxebarria,StephanieAtkins,FrancesTurrell,JyotiChhetri,NoraBengoa-Vergniory,ChristophZenzmaier,PeterBerger,JonathanWaxman,andRobertKypta
Carcinogenesis,Nov2015;10.1093/carcin/bgv153
http://carcin.oxfordjournals.org/cgi/content/abstract/bgv153v2

MELK-T1,asmall-moleculeinhibitorofproteinkinaseMELK,decreasesDNA-damagetoleranceinproliferatingcancercells
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“CombinedEffectsofSuberoylanilideHydroxamicAcidandCisplatinonRADIationSensitivityandCancerCellInvasioninNon–SmallCellLungCancer”
JianguoFeng,ShirongZhang,KanWu,BingWang,JeffreyY.C.Wong,HongJiang,RujunXu,LishaYing,HaixiuHuang,XiaoliangZheng,XufengChen,andShenglinMa
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