>
产品中心 >
Other_natural_proteins >
Trevigen/Cultrex®；基底膜基质，类型2/3532-001-02/1 ml，2 x 5 ml，5 ml
Description
Cultrex®BasementMembraneExtract(BME)isasolubleformofbasementmembranepurifiedfromEngelbreth-Holm-Swarm(EHS)tumor.Thisextractprovidesanaturalextracellularmatrixhydrogelthatpolymerizesat37°Ctoformareconstitutedbasementmembrane.Basementmembranesarecontinuoussheetsofspecializedextracellularmatrixthatformaninterfacebetweenendothelial,epithelial,muscle,orneuronalcellsandtheiradjacentstromaandthatplayanessentialroleintissueorganizationbyinfluencingcelladhesion,migration,proliferation,anddifferentiation.ThemajorcomponentsofBMEincludelaminin,collagenIV,entactin,andheparansulfateproteoglycans.
Cultrex®BMEType2isaBasementMembraneMatrixthathasbeendeveloped,producedandqualifiedspecificallyforrobustorganoidcultures.
Qualitycontrolspecifications:
- Mousecoloniesareroutinelyscreenedforpathogensusing mouseantibodyproduction(MAP)testing.
- Engelbreth-Holm-Swarm(EHS)tumorisLDEV-free.
- PathClear®–testednegativebyPCRfor17bacterialandvirusstrainstypicallyincludedinmouseantibodyproduction(MAP)testing,plus13additionalmurineinfectiousagentsincludingLDEV,foratotalof31organismsandviruses.
- Nobacterialorfungalgrowthdetectedafterincubationat37°Cfor14daysfollowingUSPsterilitytestingguidelines.
- Endotoxinconcentration≤8EU/mlbyLimulusAmoebocyteLysate(LAL)assay.
- Proteinconcentrationof12–18mg/ml.
- NomycoplasmacontaminationdetectedbyPCR.
- ProvidedinDulbecco’sModifiedEagle’smedium(DMEM)withoutphenolred,with10µg/mlgentamicinsulfate.
- GelstABIlitytestedforaperiodof14daysat37°C.
- BIOLOGicalactivitydeterminedforeachlot:
- Tubeformationassay–BMEpromotesformationofcapillary-likestructuresbyhuman(HBMVEC;HUVEC)ormouse(SVEC4-10)endothelialcells.
- Organoidculture:BasementMembraneExtractpromotesgrowthandexpansionofmousesmallintestineorganoidProgenitorcells.
| Catalog#3532-010-01includes: | ||
| CatalogNumber | Description | Qty |
| 3532-005-01 | CultrexBasementMembraneExtractType2,PathClear® | 2 |
“BasementMembrane-BasedGlucoseSensorCoatingsEnhance
ContinuousGlucoseMonitoringinVivo”
UlrikeKlueh,YiQiao,CarolineCzajkowski,IzabelaLudzinska,OmarAntar,andDonaldL.Kreutzer
JournalofDiabetesScienceandTechnology,Sep2015;9:957–965.
http://dst.sagepub.com/cgi/content/abstract/9/5/957
