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Trevigen/Cultrex®;大鼠I型胶原,低粘度(3 mg/ml)/3443-003-01/1 ml,35 ml
Description
Cultrex®RatCollagenI,LowerViscosityispurifiedfromrattailtendons and isprovidedatastandard concentrationthatislowerinviscosity,makingiteasiertoworkwith.CollagenIisthemajorstructuralcomponentofextracellularmatrices(ECM)foundinconnectivetissueandinternalorgans,butismostprevalentinthedermis,tendons,andbone.Itisa300kDamoleculecomposedoftwoalpha1(I)chainsandonealpha2(I)chainthatspontaneouslyformsatriplehelixscaffoldataneutralpHand37°C.Thisphenomenoncanbeexploitedtopromotecellattachment,growth,differentiation,migration,andtissuemorphogenesisduringdevelopment.ToprovidethemoststandardizedCollagenI,aspecialprocessisemployedtoprovidematerialatastandardconcentrationofapproximately3mg/ml.
Cultrex®RatCollagenI,LowerViscosityisapurifiedstromal ECMprotein thathasbeendeveloped,producedandqualifiedatalowviscosityforgeneralcellcultureapplications.
Qualitycontrolspecifications:
- Nobacterialorfungalgrowthdetectedafterincubationat37°Cfor14daysfollowingUSPsterilitytestingguidelines.
- Endotoxinconcentration≤20EU/mlbyLimulusAmoebocyteLysate(LAL)assay.
- NomycoplasmacontaminationdetectedbyPCR.
- Proteinconcentrationof3mg/ml.
- Providedin 20mMAceticAcid,pH3.4–3.6.
- CollagenIformsagelwhendilutedto0.4mg/mlatneutralpH.
- BIOLOGicalactivitydeterminedforeachlot: