Description

Cultrex®BovineCollagenI ispurifiedfromfetalbovineextensor tendons and ispepsin-treatedtoremovetelopeptides.CollagenIisthemajorstructuralcomponentofextracellularmatrices(ECM)foundinconnectivetissueandinternalorgans,butismostprevalentinthedermis,tendons,andbone.Itisa300kDamoleculecomposedoftwoalpha1(I)chainsandonealpha2(I)chainthatspontaneouslyformsatriplehelixscaffoldataneutralpHand37°C.Thisphenomenoncanbeexploitedtopromotecellattachment,growth,differentiation,migration,andtissuemorphogenesisduringdevelopment.ToprovidethemoststandardizedCollagenI,aspecialprocessisemployedtoprovidematerialatastandardconcentrationofapproximately5mg/mL.

Cultrex®BovineCollagenI isapurifiedstromalECMprotein thathasbeendeveloped,producedandqualifiedforgeneralcellcultureapplications.

Qualitycontrolspecifications:

• Nobacterialorfungalgrowthdetectedafterincubationat37°Cfor14daysfollowingUSPsterilitytestingguidelines.
• Endotoxinconcentration≤20EU/mlbyLimulusAmoebocyteLysate(LAL)assay.
• NomycoplasmacontaminationdetectedbyPCR.
• Proteinconcentrationof5mg/ml.
• Providedin 20mMAceticAcid,pH3.4–3.6.
• CollagenIformsagelwhendilutedto0.4mg/mlatneutralpH.
• BIOLOGicalactivitydeterminedforeachlot:
  • CellAttachment:TestedfortheABIlitytopromotecellattachmentandspreADIngofHT1080humanfibrosarcomacells.

FunctionallydistinctPI3-kinasepathwaysregulatemyelinationintheperipheralnervoussystem
Heller,B.A.,Ghidinelli,M.,Voelkl,J.,Einheber,S.,Smith,R.,Grund,E.,Morahan,G.,Chandler,D.,Kalaydjieva,L.,Giancotti,F.,King,R.H.,Fejes-Toth,A.N.,Fejes-Toth,G.,Feltri,M.L.,Lang,F.andSalzer,J.L.
J.CellBiol.,Mar2014;204:1219–1236.
http://jcb.rupress.org/cgi/content/abstract/204/7/1219

Humanendothelialcolony-formingcellsserveastrophicmediatorsformesenchymalstemcellengraftmentviaparacrinesignaling
Ruei-ZengLin,RafaelMoreno-Luna,DanLi,Shou-ChingJaminet,ArinK.Greene,andJuanM.Melero-Martin
PNAS,Jun2014;10.1073/pnas.1405388111.
http://www.pnas.org/cgi/content/abstract/1405388111v1

PivotalRoleforCD16+MonocytesinImmuneSurveillanceoftheCentralNervousSystem
AnneWaschbisch,SinaSchröder,DanaSchraudner,LauraSammet,BabetteWeksler,ArthurMelms,SabinePfeifenbring,ChristineStadelmann,StefanSchwab,andRalfA.Linker
J.Immunol.,Feb2016;196:1558–1567.
http://www.jimmunol.org/cgi/content/abstract/196/4/1558