Description

Cultrex®Rat CollagenI ispurifiedfromrattailtendons and isprovidedatahighconcentrationthatiscapableofpolymerizingtoforma hydrogel.CollagenIisthemajorstructuralcomponentofextracellularmatrices(ECM)foundinconnectivetissueandinternalorgans,butismostprevalentinthedermis,tendons,andbone.Itisa300kDamoleculecomposedoftwoalpha1(I)chainsandonealpha2(I)chainthatspontaneouslyformsatriplehelixscaffoldataneutralpHand37̊C.Thisphenomenoncanbeexploitedtopromotecellattachment,growth,differentiation,migration,andtissuemorphogenesisduringdevelopment.ToprovidethemoststandardizedCollagenI,aspecialprocessisemployedtoprovidematerialatastandardconcentrationofapproximately5mg/mL.

Cultrex®Rat CollagenI isapurifiedstromalECMprotein thathasbeendeveloped,producedandqualifiedforgeneralcellcultureapplications.

Qualitycontrolspecifications:

• Nobacterialorfungalgrowthdetectedafterincubationat37°Cfor14daysfollowingUSPsterilitytestingguidelines.
• Endotoxinconcentration≤20EU/mlbyLimulusAmoebocyteLysate(LAL)assay.
• NomycoplasmacontaminationdetectedbyPCR.
• Proteinconcentrationof5mg/ml.
• Providedin 20mMAceticAcid,pH3.4–3.6.
• CollagenIformsagelwhendilutedto0.4mg/mlatneutralpH.
• BIOLOGicalactivitydeterminedforeachlot:
  • CellAttachment:TestedfortheABIlitytopromotecellattachmentandspreADIngofHT1080humanfibrosarcomacells.

GlycosylationatAsn211RegulatestheActivationStateoftheDiscoidinDomainReceptor1(DDR1)
Fu,H.L.,Valiathan,R.R.,Payne,L.,Kumarasiri,M.,Mahasenan,K.V.,Mobashery,S.,Huang,P.andFridman,R.
J.Biol.Chem.,Mar2014;289:9275–9287.
http://www.jbc.org/cgi/content/abstract/289/13/9275

RPTPαcontrolsepithelialadherensjunctions,linkingE-cadherinengagementtoc-Src-mediatedphosphorylationofcortactin
MartaTruffi,VéroniqueDubreuil,XuanLiang,NathalieVacaresse,FabienneNigon,SiewPingHan,AlphaS.Yap,GuillermoA.Gomez,andJanSap
J.CellSci.,Jun2014;127:2420–2432.
http://jcs.biologists.org/cgi/content/abstract/127/11/2420

BiphasicModulationofParacellularClaudin-5ExpressioninMouseBrainEndothelialCellsIsMediatedthroughthePhosphoinositide-3-Kinase/AKTPathway
RyanB.Camire,HollyJ.Beaulac,StephanieA.Brule,AnnieI.McGregor,EmilyE.Lauria,andColinL.Willis
J.Pharmacol.Exp.Ther.,Oct2014;351:654–662.
http://jpet.aspetjournals.org/cgi/content/abstract/351/3/654

DOCK8regulateslymphocyteshapeintegrityforskinantiviralimmunity
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http://jem.rupress.org/cgi/content/abstract/jem.20141307v1

“MaspinExpressioninProstateTumorCellsAvertsStemnessand
StratifiesDrugSensitivity”
“M.MargaridaBernardo,AlexanderKaplun,SijanaH.Dzinic,XiaohuaLi,JonathanIrish,AdelinaMujagic,
BenjaminJakupovic,JessicaB.Back,EricVanBuren,XiangHan,IvoryDean,YongQ.Chen,ELISAbeth
Heath,WaelSakr,andShijieSheng”
CancerRes.,Sep2015;75:3970–3979.
http://cancerres.aacrjournals.org/cgi/content/abstract/75/18/3970

MesencephalicAstrocyte–DerivedNeurotrophicFactorasaUrineBioMarkerforEndoplasmicReticulumStress–RelatedKidneyDiseases
YeawonKim,HeedooLee,ScottR.Manson,MariaLindahl,BradleyEvans,JeffreyH.Miner,FumihikoUrano,andYingMaggieChen
J.Am.Soc.Nephrol.,Mar2016;10.1681/ASN.2014100986
http://jasn.asnjournals.org/cgi/content/abstract/ASN.2014100986v1