Description
Cultrex®BasementMembraneExtract(BME)isasolubleformofbasementmembranepurifiedfromEngelbreth-Holm-Swarm(EHS)tumor.Thisextractprovidesanaturalextracellularmatrixhydrogelthatpolymerizesat37°Ctoformareconstitutedbasementmembrane.Basementmembranesarecontinuoussheetsofspecializedextracellularmatrixthatformaninterfacebetweenendothelial,epithelial,muscle,orneuronalcellsandtheiradjacentstromaandthatplayanessentialroleintissueorganizationbyinfluencingcelladhesion,migration,proliferation,anddifferentiation.ThemajorcomponentsofBMEincludelaminin,collagenIV,entactin,andheparansulfateproteoglycans.
Cultrex®3DCultureMatrix®RGFBMEisaBasementMembraneMatrixthathasbeendeveloped,producedandqualifiedspecificallyforusein3Dculturestudies.
Qualitycontrolspecifications:
- Mousecoloniesareroutinelyscreenedforpathogensusing mouseantibodyproduction(MAP)testing.
- Engelbreth-Holm-Swarm(EHS)tumorisLDEV-free.
- PathClear®–testednegativebyPCRfor17bacterialandvirusstrainstypicallyincludedinmouseantibodyproduction(MAP)testing,plus13additionalmurineinfectiousagentsincludingLDEV,foratotalof31organismsandviruses.
- Nobacterialorfungalgrowthdetectedafterincubationat37°Cfor14daysfollowingUSPsterilitytestingguidelines.
- Endotoxinconcentration≤8EU/mlbyLimulusAmoebocyteLysate(LAL)assay.
- Proteinconcentrationof14–16mg/ml.
- NomycoplasmacontaminationdetectedbyPCR.
- ProvidedinDulbecco’sModifiedEagle’smedium(DMEM)withoutphenolred,with10µg/mlgentamicinsulfate.
- GelstABIlitytestedforaperiodof14daysat37°C.
- BIOLOGicalactivitydeterminedforeachlot:
- Tubeformationassay–BMEpromotesformationofcapillary-likestructuresbyhuman(HBMVEC;HUVEC)ormouse(SVEC4-10)endothelialcells.
- 3DCulture:BasementMembraneExtractpromotesdifferentiationofahumanepithelialcelllinederivedfrommammarygland(MCF-10A)orhumanprostate(PC-3)intoacinarstructures.
| Catalog#3445-010-01includes: | ||
| CatalogNumber | Description | Qty |
| 3445-005-01 | 3-DCultureMatrix™ReducedGrowthFactorBasementMembraneExtract,PathClear® | 2 |
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