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Trevigen/PARP Universal Chemiluminescent Assay Kit/4676-096-K/96 tests
Description
ThisELISAbasedassaydetectsbiotinylatedpoly(ADP-ribose)depositedbyPARP-1ontoimmobilizedhistonesina96-wellformat.TheadditionofStrep-HRP(biotin-bindingprotein)andachemiluminescentHRPsubstrateyieldsrelativelightunits(RLU)thatcorrelateswithPARP-1activity.
FEATURES:
- Chemiluminescentreadout
- 96strip-wellformat
- Sensitive–detects10mUPARP/well
- AssayTime~3hrs
APPLICATIONS:
FortheinvitroscreeningofcandidatePARP-1inhibitorsanddeterminationofIC50values.
| Catalog#4676-096-Kincludes: | ||
| CatalogNumber | Description | Qty |
| 4667-50-03 | 3-Aminobenzamide | 1 |
| 4668-050-01 | HumanPARP1Enzyme,HSA | 1 |
| 4671-096-02 | 20XPARPBuffer | 2 |
| 4671-096-03 | 10XPARPCocktail | 1 |
| 4671-096-06 | 10XActivatedDNA | 1 |
| 4671-096-04 | 10XStrep-Diluent | 1 |
| 4675-096-01 | PeroxyGlow™A | 1 |
| 4675-096-02 | PeroxyGlow™B | 1 |
| 4678-096-P | Histone-CoatedWhiteStripWellPlate | 1 |
| 4800-30-06 | Strep-HRP | 1 |
RoleforDNADamageSignalinginPulmonaryArterialHypertension
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Circulation,Feb2014;129:786–797
http://circ.ahajournals.org/cgi/content/abstract/129/7/786
YU238259IsaNovelInhibitorofHomology-DependentDNARepairThatExhibitsSyntheticLethalityandRADIosensitizationinRepair-DeficientTumors
GregoryC.Stachelek,ElizabethPeterson-Roth,YanfengLiu,RafaelJ.Fernandez,III,LukeR.G.Pike,JackM.Qian,LauraAbriola,DentonHoyer,WilliamHungerford,JanieMerkel,andPeterM.Glazer
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http://mcr.aacrjournals.org/cgi/content/abstract/13/10/1389
“LatonduineAnalogsRestoreF508del–CysticFibrosisTransmembrane
ConductanceRegulatorTraffickingthroughtheModulationofPoly-ADP
RibosePolymerase3andPoly-ADPRibosePolymerase16Activity”
“GraemeW.Carlile,RenaudRobert,ElizabethMatthes,QiYang,RobertoSolari,RichardHatley,ColinM.
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