Description
ThisELISAbasedassaydetectsbiotinylatedpoly(ADP-ribose)depositedbyPARP-1ontoimmobilizedhistonesina96-wellformat.TheadditionofStrep-HRP(biotin-bindingprotein)andacolorimetricHRPsubstrateyieldsrelativeabsorbancethatcorrelateswithPARP-1activity.
FEATURES:
- Colorimetricreadout
- 96strip-wellformat
- Sensitive–detects10mUPARP/well
- AssayTime~3hrs
APPLICATIONS:
FortheinvitroscreeningofcandidatePARP-1inhibitorsanddeterminationofIC50values.
| Catalog#4677-096-Kincludes: | ||
| CatalogNumber | Description | Qty |
| 4667-50-03 | 3-Aminobenzamide | 1 |
| 4668-050-01 | PARP-HSA,10Units/µl | 1 |
| 4671-096-02 | 20XPARPBuffer | 2 |
| 4671-096-03 | 10XPARPCocktail | 1 |
| 4671-096-06 | 10XActivatedDNA | 1 |
| 4671-096-04 | 10XStrep-Diluent | 1 |
| 4677-096-P | Histone-CoatedPlateNaturalStripWellPlate | 1 |
| 4800-30-06 | Strep-HRP | 1 |
| 4822-96-08 | TACS-Sapphire™ | 1 |
EffectofSmall-MoleculeModificationonSingle-CellPharmacokineticsofPARPInhibitors
GregM.Thurber,ThomasReiner,KatherineS.Yang,RainerH.Kohler,andRalphWeissleder
Mol.CancerTher.,Apr2014;13:986–995
http://mct.aacrjournals.org/cgi/content/abstract/13/4/986
CombinatorialEffectsofPARPInhibitorPJ34andHistoneDeacetylaseInhibitorVorinostatonLeukemiaCellLines
EWAJASEK,MARIUSZGAJDA,GRZEGORZJ.LIS,MAŁGORZATAJASIŃSKA,andJANA.LITWIN
AnticancerRes,Apr2014;34:1849–1856.
http://ar.iiarjournals.org/cgi/content/abstract/34/4/1849
Silencingofpoly(ADP-ribose)polymerase-1suppresseshyperstretch-inducedexpressionofinflammatorycytokinesinvitro
Wang,J.,Liu,L.,Xia,Y.andWu,D.
ActaBiochimBiophysSin,May2014;10.1093/abbs/gmu035.
http://abbs.oxfordjournals.org/cgi/content/abstract/gmu035v1
NewInsightsintoPARPInhibitors’EffectonCellCycleandHomology-DirectedDNADamageRepair
PetarJelinicandDouglasA.Levine
Mol.CancerTher.,Jun2014;13:1645–1654.
http://mct.aacrjournals.org/cgi/content/abstract/13/6/1645
DisruptionofWnt/β-CateninSignalingandTelomericShorteningAreInextricableConsequencesofTankyraseInhibitioninHumanCells
“OzlemKulak,HuaChen,BrodyHolohan,XiaofengWu,HuaweiHe,DominikaBorek,Zbyszek
Otwinowski,KiyoshiYamaguchi,LaurenA.Garofalo,ZhiqiangMa,WoodringWright,ChuoChen,JerryW.Shay,XuewuZhang,andLawrenceLum”
Mol.Cell.Biol.,Jul2015;35:2425–2435.
http://mcb.asm.org/cgi/content/abstract/35/14/2425
Acyl-CoA-bindingdomaincontaining3modulatesNAD+metabolismthroughactivatingpoly(ADP-ribose)polymerase1
YongChen,SookheeBang,SoohyunPark,HanyuanShi,andSangwonF.Kim
Biochem.J.,Jul2015;469:189–198.
http://www.biochemj.org/cgi/content/abstract/469/2/189
