Description
ImmobilizedPARmonoclonalantibodyinthewellsofa96-wellplatecapturescellularPARandPARattachedtoproteins. IncubationwithapolyclonalPARdetectingantibody,followedbyadditionofagoatanti-rabbitIgG-HRPsecondaryandachemiluminescentHRPsubstrateyieldsrelativelightunits(RLU)thatdirectlycorrelateswiththeamountofcellularPAR.
FEATURES:
- Pre-coated96wellcaptureantibodyplates
- Highsignaltonoiseratio
- Detectionsensitivityof2pg/mlofPAR
- Broadlineardynamicrangeto1,000pg/ml
- Reducedinter-assayvariABIlity
- ValidatedassaythatmeasuresdrugactiononPARPinbothinvivoandinvitrosettings
- 96testsize
APPLICATIONS:
- QuantitationofPARinperipheralbloodmononuclearcells,tissueculturecells,andtumorlysatesfromdifferenttissues,organsandxenografts.
- MonitoringtheefficacyofPARPinhibitorsonPARformationinvivo.
- VerifyingobservationsofenhancedcancercellcytotoxicityarisingfromPARPinhibitor/anticancerdrugcombinationtherapy.
- FacilitatingdevelopmentofPARPandPARGtargetedtherapeutics.
| Catalog#4520-096-Kincludes: | ||
| CatalogNumber | Description | Qty |
| 4520-096-01 | PARStandard,25pg/µl | 1 |
| 4520-096-08 | JurkatCellLysateControlStandard,Low | 1 |
| 4520-096-09 | JurkatCellLysateControlStandard,Medium | 1 |
| 4520-096-10 | JurkatCellLysateControlStandard,High | 1 |
| 4520-096-03 | PARPolyclonalDetectingAntibody | 1 |
| 4520-096-04 | Goatanti-RabbitIgG-HRP | 1 |
| 4520-096-06 | DNaseI,2Units/µl | 1 |
| 4520-096-02 | SampleBuffer | 1 |
| 4520-096-05 | CellLysisReagent | 1 |
| 4520-096-07 | 100XMagnesiumCation | 1 |
| 4520-096-11 | AntibodyDiluent | 1 |
| 4520-096-12 | 20%SDS | 1 |
| 4520-096-P | Pre-coatedwhite96-stripwellplateand5sealers | 1 |
| 4675-096-01 | PARPPeroxyGlow™A | 1 |
| 4675-096-02 | PARPPeroxyGlow™B | 1 |
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