Description
ThisELISAbasedassaydetectspoly(ADP-ribose)depositedbyPARP-1ontoimmobilizedhistonesina96-wellformat.Ananti-PARmonoclonaldetectingantibodyfollowedbyadditionofagoatanti-rabbitIgG-HRPsecondaryandacolorimetricHRPsubstrateyieldsrelativeabsorbancethatcorrelateswithPARP-1activity. DuringapoptosisPARP-1iscleavedwitharesultingdropinactivity.Etoposideisincludedasacontrolapoptosisinducer.
FEATURES:
- Colorimetricreadout
- 96wellformat
- Highlysensitive–detects0.1mUPARP~500cells
- Dynamicrangebetween0.1to10mUPARP
- Requires10-100ngextractfordetection
- AssayTime~3hrs
APPLICATIONS:
- MeasurePARP-1activityincellextractsbeforeandafterapoptosis.
- ForthescreeningofcandidatePARP-1inhibitorsanddeterminationofIC50values.
| Catalog#4684-096-Kincludes: | ||
| CatalogNumber | Description | Qty |
| 4671-096-06 | 10XActivatedDNA | 1 |
| 4684-096-01 | PARP-HSA,10mUnits/µl | 1 |
| 4684-096-02 | 20mMNAD | 1 |
| 4684-096-04 | Anti-PARmonoclonalAntibody | 1 |
| 4684-096-05 | Goatanti-mouseIgG-HRP | 1 |
| 4684-096-06 | 10mMEtoposide | 1 |
| 4684-096-07 | 20XI-PARAssayBuffer | 1 |
| 4684-096-03 | 5XAntibodyDiluent | 1 |
| 4684-096-P | Histone-CoatedNaturalStripWellPlate,I-PAR | 1 |
| 4822-96-08 | TACS-Sapphire | 1 |
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