Description

CONCENTRATION
10 units/µl
SOURCE
Purified from E.  coli containing a recombinant plasmid harboring human PARP1 gene.
SUBSTRATE SPECIFICITY
PARP1 uses NAD⁺ as a substrate to catalyze poly(ADP-ribosyl)ation of target proteins involved  in  chromatin  structure  and  DNA  metabolism. PARP1 is stimulated by DNA-strand breaks and capable of auto-ribosylation.
STORAGE
Store at -20°C in a manual defrost freezer. For long-term storage, freeze at -80°C in working aliquots.
APPLICATIONS
Identification of inhibitors of PARP1 activity
Quantitation of DNA Damage
Investigation of PARP1 inactivation during apoptosis
Western Blot Analysis

Poly(ADP-ribose) polymerase-dependent energy depletion occurs through inhibition of glycolysis
Shaida A. Andrabi, George K. E. Umanah, Calvin Chang, Daniel A. Stevens, Senthilkumar S. Karuppagounder, Jean-Philippe Gagné, Guy G. Poirier, Valina L. Dawson, and Ted M. Dawson
PNAS, Jul 2014; 111: 10209 – 10214.
http://www.pnas.org/cgi/content/abstract/111/28/10209

The N-terminal Region of Chromodomain Helicase DNA-binding Protein 4 (CHD4) Is Essential for Activity and Contains a High Mobility Group (HMG) Box-like-domain That Can Bind Poly(ADP-ribose)
Ana P. G. Silva, Daniel P. Ryan, Yaron Galanty, Jason K. K. Low, Marylene Vandevenne, Stephen P. Jackson, and Joel P. Mackay
J. Biol. Chem., Jan 2016; 291: 924 – 938.
http://www.jbc.org/cgi/content/abstract/291/2/924

Disruption of Macrodomain Protein SCO6735 Increases Antibiotic Production in Streptomyces coelicolor
“Jasna Lalic, Melanija Posavec Marjanovic, Luca Palazzo, Dragutin Perina, Igor Sabljic, Roko Zaja, Thomas
Colby, Bruna Plese, Mirna Halasz, Gytis Jankevicius, Giselda Bucca, Marijan Ahel, Ivan Matic, Helena Cetkovic, Marija Luic, Andreja Mikoc, and Ivan Ahel”
J. Biol. Chem., Oct 2016; 291: 23175 – 23187
http://www.jbc.org/cgi/content/abstract/291/44/23175

Poly(ADP-ribose) polymerases covalently modify strand break termini in DNA fragments in vitro
“Ibtissam Talhaoui, Natalia A. Lebedeva, Gabriella Zarkovic, Christine Saint-Pierre, Mikhail M. Kutuzov,
Maria V. Sukhanova, Bakhyt T. Matkarimov, Didier Gasparutto, Murat K. Saparbaev, Olga I. Lavrik, and
Alexander A. Ishchenko”
Nucleic Acids Res., Nov 2016; 44: 9279 – 9295
http://nar.oxfordjournals.org/cgi/content/abstract/44/19/9279

PARP1-dependent recruitment of KDM4D histone demethylase to DNA damage sites promotes double-strand break repair
Khoury-Haddad, H., Guttmann-Raviv, N., Khoury-Haddad, H., Ipenberg, I., Guttmann-Raviv, N., Huggins, D., Ipenberg, I., Jeyasekharan, A.D., Huggins, D., Ayoub, N., Jeyasekharan, A.D., and Ayoub, N.,
PNAS, Feb 2014; 111: E728 – E737.
http://www.pnas.org/cgi/content/abstract/111/7/E728

Differential and Concordant Roles for Poly(ADP-Ribose) Polymerase 1 and Poly(ADP-Ribose) in Regulating WRN and RECQL5 Activities
Prabhat Khadka, Joseph K. Hsu, Sebastian Veith, Takashi Tadokoro, Raghavendra A. Shamanna, Aswin Mangerich, Deborah L. Croteau, and Vilhelm A. Bohr
Mol. Cell. Biol., Dec 2015; 35: 3974 – 3989.
http://mcb.asm.org/cgi/content/abstract/35/23/3974