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含10倍REC的Trevigen/DPO-4聚合酶™ 缓冲液15和阳离子/4150-010-EB/10μg、1ml、50μg、1ml
Description
CONCENTRATION
10or50µgpervial
SOURCE
PurifiedfromE.colicontainingarecombinantplasmidharboringtheS.solfataricusDpo4gene.
SUBSTRATESPECIFICITY
TranslesionsynthesisacrossAPsites,cis-synthymine-thyminedimer,6-4photoproducts,cisplatinum-andacetylaminofluoreneguanineadducts.
STORAGE
Storeat-20°Cinamanualdefrostfreezer.Forlong-termstorage,freezeat-80°Cinworkingaliquots.
APPLICATIONS
AllowsforcouplingtoPCRforamplificationofdamagedDNA.
| Catalog#4150-050-EBincludes: | ||
| CatalogNumber | Description | Qty |
| 3900-500-15 | 10XREC™Buffer15 | 1 |
| 4150-010-02 | 100mMMgCl2 | 1 |
| 4150-010-03 | 50mMMnCl2 | 1 |
| 4150-050-01 | S.solfataricusDpo4 | 1 |
| Catalog#4150-010-EBincludes: | ||
| CatalogNumber | Description | Qty |
| 3900-500-15 | 10XREC™Buffer15 | 1 |
| 4150-010-02 | 100mMMgCl2 | 1 |
| 4150-010-03 | 50mMMnCl2 | 1 |
| 4150-010-01 | S.solfataricusDpo4 | 1 |
StructuralandbiochemicalimpactofC8-aryl-guanineadductswithintheNarIrecognitionDNAsequence:influenceofarylringsizeontargetedandsemi-targetedmutagenicity
MichaelSproviero,AnneM.R.Verwey,KatherineM.Rankin,AaronA.Witham,DmitriyV.Soldatov,RichardA.Manderville,MostafaI.Fekry,ShanaJ.Sturla,PurshotamSharma,andStaceyD.WetmoreNucleicAcidsRes.,Dec2014;42:13405–13421.
NucleicAcidsRes.,Dec2014;42:13405–13421
http://nar.oxfordjournals.org/cgi/content/abstract/42/21/13405
