Description

Organoidculturesexhibitcellularbehaviorsandmorphologiessimilartothoseseeninvivo,however,theadaptationofthesemodelsforstudyingbiochemicalprocesseshasbeenimpededbythechallengeofseparatingintactorganoidsfromextra-cellularproteinscomprisingthehydrogel.Commonly,proteasesareemployedtodegradetheseextracellularproteins,however,proteasesalsodegradeproteinsonthecellsurfaceandproteaseactivitymaycarryoverintosubsequentculturesorlysatepreparations.Trevigen’sCultrex^®OrganoidHarvestingSolutionprovidesanon-enzymaticmethodfordepolymerizingextracellularmatrixproteinstoallowforharvestingofintactorganoidsforpassaging,cryopreservation,orbiochemicalanalysis.

Qualitycontrolspecifications:

• Functionalassay–TenvolumesofOrganoidHarvestingSolution(500μl)depolymerizesa50μldomeofbasementmembranematrixina24wellplatewithmoderateshakingonicefor90minutes.

• Sterilitytesting–Nobacterialorfungalgrowthdetectedafterincubationat37°Cfor14days.

• Endotoxinconcentration≤20EU/mlbyLALassay.

StorageConditions:

Productisstableforatleast6monthsfromthedateofreceiptwhenstoredat2–8°C.Keepsterile.