Description

Cultrex®StemCellQualifiedHumanFibronectin,Pathclear®ispurifiedfromhumanplasmaandisPathclear®certifiedtobefreeofcommonpathogens.Fibronectinisanextracellularmatrixproteinthatisfoundabundantlyinbloodandconnectivetissues.Thesematricesareassociatedwiththeepithelialtomesenchymaltransitionofmetastaticcells,includingtumorcellswithstemcell-likeproperties.Fibronectinperformsessentialfunctionsincollagenfibrillogenesis,aseitherageneralcelladhesionmoleculeoramodulatorinbindingbetweencellsurfacesandtheextracellularmatrix.Fibronectinmatrixassemblyisessentialfornormalvertebratedevelopmentandapparentlycontributestothegenerationoftumormetastasesbysupportingtheestablishmentandpersistenceofpre-metastaticniches.Fibronectinissecretedasadisulfide-linkeddimerof230-270kDa,comprisedofthreetypesofrepeatingmodulesthatmediateinteractionswithextracellularmatrixcomponents(includingfibronectinitself),andcellsviaintegrinsandotherfibronectinreceptors.Thus,fibronectincanbeusedforcoatingtissueculturesurfacesorasamediumadditivetopromotecelladhesionandproliferation;itis0.2μmsterilefiltered.

Cultrex®StemCellQualifiedHumanFibronectin,Pathclear®isapurifiedplasmaextracellularmatrix proteinthathasbeendeveloped,producedandqualifiedspecifically toprovideafunctionallydefinedandeffectivefeeder-freesurfacefortheattachmentandmaintenanceofembryonicstemcellsinapluripotentstate

Qualitycontrolspecifications:

• PathClear®–testednegativeforeighthumanpathogenicvirusesincludingHepatitisA,BandC,HIV1and2,Hantaan,Seoul,andSinNombrebyPCR.
• Nobacterialorfungalgrowthdetectedafterincubationat37°Cfor14daysfollowingUSPsterilitytestingguidelines.
• Endotoxinconcentration≤20EU/mlbyLimulusAmoebocyteLysate(LAL)assay.
• Proteinconcentrationof1mg/ml.
• NomycoplasmacontaminationdetectedbyPCR.
• Providedin100mMCAPS,150mMNaCl,1mMCaCI2,pH11.5.
• Purity ≥90%(SDS-PAGE).
• BIOLOGicalactivitydeterminedforeachlot:
  • PromotestheattachmentofhumaniPSCs.
  • EffectivelymaintainshumaniPSCsinapluripotentstateinafeeder-freeculture.

SafetyStatement:

AlthoughCultrex®StemCellQualifiedHumanFibronectin,PathClear®hasbeentestedforthevirusesaboveitcontainshumansourcematerialandthereforeshouldbetreatedaspotentiallyinfectiousandhandledattheBiologicalSafetyLevel2tominimizeexposure.