4000-520-616
欢迎来到免疫在线!(蚂蚁淘生物旗下平台)  请登录 |  免费注册 |  询价篮
主营:主营:分子类,蛋白类,抗体类,生化类试剂
咨询热线电话
4000-520-616
当前位置: 首页 > 产品中心 > Additives > Trevigen/Cultrex®;3D培养基质大鼠胶原I/3447-020-01/20 ml
商品详细Trevigen/Cultrex®;3D培养基质大鼠胶原I/3447-020-01/20 ml
Trevigen/Cultrex®;3D培养基质大鼠胶原I/3447-020-01/20 ml
Trevigen/Cultrex®;3D培养基质大鼠胶原I/3447-020-01/20 ml
商品编号: 3447-020-01
品牌: Trevigen Inc
市场价: ¥3300.00
美元价: 1980.00
产地: 美国(厂家直采)
公司:
产品分类: 添加物
公司分类: Additives
联系Q Q: 3392242852
电话号码: 4000-520-616
电子邮箱: info@ebiomall.com
商品介绍

Description

Cultrex®3DCultureMatrix®CollagenI ispurifiedfromrattailtendons and isprovidedatahighconcentrationthatiscapableofpolymerizingtoforma hydrogel.CollagenIisthemajorstructuralcomponentofextracellularmatrices(ECM)foundinconnectivetissueandinternalorgans,butismostprevalentinthedermis,tendons,andbone.Itisa300kDamoleculecomposedoftwoalpha1(I)chainsandonealpha2(I)chainthatspontaneouslyformsatriplehelixscaffoldataneutralpHand37̊C.Thisphenomenoncanbeexploitedtopromotecellattachment,growth,differentiation,migration,andtissuemorphogenesisduringdevelopment.ToprovidethemoststandardizedCollagenIforusein3-Dcultures,aspecialprocessisemployedtoprovidematerialatastandardconcentrationofapproximately5mg/mL.Thismaterialisthenincorporatedina3-Dculturetovalidateefficacy.

Cultrex®3DCultureMatrix®CollagenI isapurifiedstromalECMprotein thathasbeendeveloped,producedandqualifiedspecificallyforusein3Dculturestudies.

Qualitycontrolspecifications:

  • Nobacterialorfungalgrowthdetectedafterincubationat37°Cfor14daysfollowingUSPsterilitytestingguidelines.
  • Endotoxinconcentration≤20EU/mlbyLimulusAmoebocyteLysate(LAL)assay.
  • NomycoplasmacontaminationdetectedbyPCR.
  • Proteinconcentrationof5mg/ml.
  • Providedin 20mMAceticAcid,pH3.4–3.6.
  • BIOLOGicalactivitydeterminedforeachlot:
    • 3-DCulture:CollagenIformsagelwhendilutedto0.4mg/mlatneutralpHandpromotesattachmentandgrowthofmurineendothelialSVEC4-10cells.
    • CellAttachment:TestedfortheABIlitytopromotecellattachmentandspreADIngofHT1080humanfibrosarcomacells.

“OrganotypicCultureofUntransformedandTumOrigenicPrimaryMammaryEpithelialCells”
MartinJechlinger
ColdSpringHarbProtoc,May2015;2015:pdb.prot078295
http://cshprotocols.cshlp.org/cgi/content/abstract/2015/5/pdb.prot078295

品牌介绍
Trevigen公司的3D和2D细胞培养产品的设计,开发,生产,合格,由我们Cultrex支持®实验室工作人员。我们的产品包括替代基质胶®以及合格的三维培养几个独特类型的基底膜提取物(BME)矩阵,干细胞化培养和球体。提供的其他基质包括层粘连蛋白I,胶原蛋白I,胶原蛋白IV,玻连蛋白,纤连蛋白,聚-D-赖氨酸和聚-L-赖氨酸。