Description
Cultrex®BovineCollagenI ispurifiedfromfetalbovineextensor tendons and ispepsin-treatedtoremovetelopeptides.CollagenIisthemajorstructuralcomponentofextracellularmatrices(ECM)foundinconnectivetissueandinternalorgans,butismostprevalentinthedermis,tendons,andbone.Itisa300kDamoleculecomposedoftwoalpha1(I)chainsandonealpha2(I)chainthatspontaneouslyformsatriplehelixscaffoldataneutralpHand37̊C.Thisphenomenoncanbeexploitedtopromotecellattachment,growth,differentiation,migration,andtissuemorphogenesisduringdevelopment.ToprovidethemoststandardizedCollagenI,aspecialprocessisemployedtoprovidematerialatastandardconcentrationof1mg/mL.
Cultrex®5X CollagenISolutionisapurifiedstromalECMprotein thathasbeendeveloped,producedandqualifiedfortheCultrex®CollagenICellInvasionAssays.
Qualitycontrolspecifications:
- Nobacterialorfungalgrowthdetectedafterincubationat37°Cfor14daysfollowingUSPsterilitytestingguidelines.
- Endotoxinconcentration≤20EU/mlbyLimulusAmoebocyteLysate(LAL)assay.
- NomycoplasmacontaminationdetectedbyPCR.
- Proteinconcentrationof1mg/ml.
- Providedin 20mMAceticAcid,pH3.4–3.6.
- CollagenIformsagelwhendilutedto0.4mg/mlatneutralpH.
- BIOLOGicalactivitydeterminedforeachlot:
