Description
DIVAA™isthefirstinvivosystemforthestudyofangiogenesisthatprovidesquantitativeandreproducIBLeresults.Duringthecourseoftheassay,implantgradesiliconecylindersclosedatoneend,calledangioreactors,arefilledwith20μlofTrevigen’sPathClear®basementmembraneextract(BME)premixedwithorwithoutangiogenic-modulatingfactors*.Theseangioreactorsarethenimplantedsubcutaneouslyinthedorsalflankofnudemice.Accompaniedwiththeonsetofangiogenesis,vascularendothelialcellsproceedtogrowintotheBMEandformvesselsintheangioreactor.Asearlyasninedayspost-implantation,thereareenoughcellstodetermineaneffectivedoseresponsetoangiogenicmodulatingfactors.Thesleekdesignoftheangioreactorprovidesastandardizedplatformforreproducibleandquantifiableinvivoangiogenesisassays.Comparedtotheplugassay,theangioreactorpreventsassayerrorsduetoabsorptionofthebasementmembraneextractbythemouse.Inaddition,theangioreactorusesonlyafractionofthematerialsconservingbothBMEandtestcompoundsused,anduptofourangioreactorsmaybeimplantedineachmouse,allowingforgreaterstatisticalpower.Trevigen’sDIVAAhasbeenusedinavarietyofinvestigations.
*ThePathClear®designationmeans:
- Nobacterialorfungalgrowthdetectedafterincubationat37°Cfor14daysfollowingUSPXXIVChapter71sterilitytest.
- NegativebyPCRtestfor:mycoplasma,17bacterialandvirusstrainstypicallyincludedinmouseantibodyproduction(MAP)testing,plus13additionalmurineinfectiousagentsincludingLDEV,foratotalof31organismsandviruses.
| Catalog#3450-048-SKincludes: | |||
| CatalogNumber | Description | Qty | |
| 3450-048-02 | DIVAABME | 1 | |
| 3450-048-04 | DIVAAFGF-2PositiveControl | 1 | |
| 3450-048-05 | DIVAACellSperse | 1 | |
| 3450-048-B10 |
| 1 | |
| 3450-048-01 | DIVAAAngioreactor | 1 | |
| 3450-048-09 |
| 1 | |
| 3450-048-03 | DIVAAWashBuffer | 1 | |
| 3450-048-06 | DIVAAFITC-Lectin200X | 1 | |
| 3450-048-07 | 25XFITC-LectinDiluent | 1 | |
| 3450-048-08 | HeparinSolution | 1 | |
“Interleukin-1ReceptorType2Actswithc-FostoEnhancethe
ExpressionofInterleukin-6andVascularEndothelialGrowthFactorA
inColonCancerCellsandInduceAngiogenesis”
“Ai-ChungMar,Chun-HoChu,Hui-JuLee,Chia-WenChien,Jing-JyCheng,Shung-HaurYang,Jeng-Kai
Jiang,andTe-ChangLee”
J.Biol.Chem.,Sep2015;290:22212–22224.
http://www.jbc.org/cgi/content/abstract/290/36/22212
