Description
3DCulturesexhibitcellularbehaviorsandmorphologiessimilartothoseseeninvivo;however,theadaptationofthesemodelsforstudyingbiochemicalprocesseshasbeenimpededbythechallengeofseparatingintactcellsfromextracellularmatrixhydrogels. Commonly,proteasesareemployedtodegradetheseextracellularproteins;however,proteasesalsodegradeproteinsonthecellsurfaceandmaycarryoverintolysatepreparations.Non-enzymaticmethodologieshavealsobeendescribedfordepolymerizing extracellularmatrixproteins,althoughtheimplementationoftheseprotocolsremainsproblematicforsomeresearchers.TheCultrex®3DCultureCellHarvestingKitdepolymerizesBMEandLamininIhydrogels,non-enzymatically,providinganoptimizedandstandardizedsolutionfortheisolationof3Dstructuresandpreparationofcelllysatesforsubsequentbiochemicalanalysis.
| CatalogNumber | Description | Qty |
|---|---|---|
| 3448-020-01 | 3-DCulture10XCellHarvestingBuffer | 1 |
| 3448-020-02 | 3-DCulture10XCellWashBuffer | 1 |
| 3448-020-03 | 3-DCultureSampleBuffer | 1 |
| 3448-020-04 | 3-DCulture10XLoADIngBuffer | 1 |
| 2275-PC-020 | HumanAnti-G3PDHAntibody | 1 |
