Description
3DCulturesexhibitcellularbehaviorsandmorphologiessimilartothoseseeninvivo;however,theadaptationofthesemodelsforstudyingbiochemicalprocesseshasbeenimpededbythechallengeofseparatingintactcellsfromextracellularmatrixhydrogels. Commonly,proteasesareemployedtodegradetheseextracellularproteins;however,proteasesalsodegradeproteinsonthecellsurfaceandmaycarryoverintolysatepreparations.Non-enzymaticmethodologieshavealsobeendescribedfordepolymerizing extracellularmatrixproteins,althoughtheimplementationoftheseprotocolsremainsproblematicforsomeresearchers.TheCultrex®3DCulture10XCellHarvestingBufferdepolymerizesBMEandLamininIhydrogels,non-enzymatically,providinganoptimizedandstandardizedsolutionfortheisolationof3Dstructuresandpreparationofcelllysatesforsubsequentbiochemicalanalysis.
