Description
IntheSuperoxideDismutaseAssay,ionsgeneratedfromtheconversionofxanthinetouricacid,andhydrogenperoxidebyxanthineoxidase(XOD),convertNBTtoNBT-diformazan.NBT-diformazanabsorbslightat550nm.SODsreducesuperoxideionconcentrationsandtherebylowertherateofNBT-diformazanformation.TheextentofreductionintheappearanceofNBT-diformazanisameasureofSODactivitypresentinexperimentalsamples.Theassayisfreeofinterferencebyothercatalyticactivities,andisidealforassayingSODinmammaliancelllysates.Thekitcontainstheproperlysisbufferandthereagentsneededfor100experimentaltests,50positivecontrols,and50negativecontrols.UnlikesomeotherassaykitsforSOD,thissystemisnotgreatlydisturbedbytracemetals.Eachassayrequiresonlyaboutfiveminutes,andafterasimplecalculation,thepercentinhibitionoftheformationofNBT-diformazanbySODisconvertedtotherelativeactivityofthesample.
FEATURES:
- Suitableformammaliancells.
- Eachsampletakesonly5minutes.
- ContainsSODfor50positivecontrols.
- Suitablefortheassayof(Mn2+)-SOD,(Fe2+)-SOD,and(Cu/Zn)–SOD.
APPLICATIONS:
CalculatingSODactivitybymeasuringthereductionofNBT-diformazanincellextracts.
| Catalog#7500-100-Kincludes: | ||
| CatalogNumber | Description | Qty |
| 7500-100-01 | SuperOxideDismutase | 1 |
| 7500-100-05 | XODSolution | 1 |
| 7500-100-02 | 25XSODReactionBuffer | 1 |
| 7500-100-03 | XanthineSolution | 1 |
| 7500-100-04 | NBTSolution | 1 |
| 7500-100-06 | 20XCellLysisSolution | 1 |
RosiglitazoneCausesCardiotoxicityviaPeroxisomeProliferator-ActivatedReceptor{gamma}-IndependentMitochondrialOxidativeStressinMouseHearts
HuameiHe,HaiTao,HuiXiong,ShengZhongDuan,FrancisX.McGowan,Jr.,RichardM.Mortensen,andJamesA.Balschi
Toxicol.Sci.,Apr2014;138:468–481.
http://toxsci.oxfordjournals.org/cgi/content/abstract/138/2/468
Protectiveactivityofanovelresveratrolanalogue,HS-1793,againstDNAdamagein137Cs-irrADIatedCHO-K1cells
Jeong,M.H.,Yang,K.M.,Jeong,D.H.,Lee,C.G.,Oh,S.J.,Jeong,S.K.,Lee,K.W.,Jo,Y.R.andJo,W.S.
J.RadiatRes,May2014;55:464–47
http://jrr.oxfordjournals.org/cgi/content/abstract/55/3/464
