Description
7512-100-k_cuvette_assay_calculation_worksheet
7512-100-k_plate_assay_calculation_worksheet
TetramericGlutathionePeroxidasecatalyzesthereductionofH2O2towater,andorganicperoxidestothecorrespondingstablealcohols,byusingglutathioneasasourceofreducingequivalents.Itrequiresseleniumasacofactorandcontainsaselenocysteineaminoacidresidueintheactivesiteofeachmonomerthatparticipatesintheactualmechanismoftheenzyme.GlutathioneReductase(GR),providedwitheachkit,thenreducestheoxidizedglutathionetocompletethecycle.TheoxidationofNADPHtoNADP+isaccompaniedbyadecreaseinabsorbanceat340nm.Therateofdecreaseintheabsorbanceat340nmisdirectlyproportionaltotheGlutathionePeroxidaseactivityinthesample.
FEATURES:
- Suitablefora96wellorcuvetteformat
- Suitableforplasma,erythrocytelysates,tissuehomogenates,andcelllysates
APPLICATIONS:
Calculatingglutathioneperoxidaseactivity.
| Catalog#7512-100-Kincludes: | ||
| CatalogNumber | Description | Qty |
| 7512-100-01 | GlutathionePeroxidase | 1 |
| 7512-100-02 | 10XAssayBuffer | 1 |
| 7512-100-03 | GSH+NADPH | 1 |
| 7512-100-04 | GlutathioneReductase | 1 |
| 7512-100-05 | CumeneHydroperoxide | 1 |
| 7512-100-06 | 96-wellplates | 1 |
Glutathione-MediatedDetoxificationofHalobenzoquinoneDrinkingWaterDisinfectionByproductsinT24Cells
JinhuaLi,WeiWang,HongquanZhang,X.ChrisLe,andXing-FangLi
Toxicol.Sci.,Oct2014;141:335–343.
http://toxsci.oxfordjournals.org/cgi/content/abstract/141/2/335
