Description
7511-100-k_calculation_worksheet
Trevigen’sGlutathioneAssayutilizesacarefullyoptimizedenzymaticrecyclingmethod,whichemploysGlutathioneReductase,forthequantificationofglutathione.ThesulfhydrylgroupofglutathionereactswithDTNB(5,5’-dithiobis-2-nitrobenzoicacid,Ellman’sreagent)andproducesayellow-colored5-thio-2-nitrobenzoicacid(TNB).Themixeddisulfide,GSTNB(betweenGSHandTNB)thatisconcomitantlyproduced,isreducedbyGlutathioneReductasetorecycletheGSHandproducemoreTNB.TherateofTNBproductionisdirectlyproportionaltotheconcentrationofGSHinthesample.Thus,measurementoftheabsorbanceofTNBat405or412nmprovidesanaccurateestimationoftheamountofglutathioneinthesample.
FEATURES:
- Suitableformammaliancells,tissue,blood,plasmaandotherbodilyfluids.
- Formattedfor96-wellplates.
- Containssufficientreagentstoassay384datapointsortodetermineGSHin:
- 123experimentalsamples,eachperformedintriplicate,plusoneGSHstandardcurve.
- 108experimentalsamples,eachperformedintriplicate,plus4GSHstandardcurves.
- 88experimentalsamples,eachperformedintriplicate,plus8GSHstandardcurves.
- Includesprocedurefordeterminingoxidizedandreducedglutathione.
APPLICATIONS:
Calculatingtotal,reduced,andoxidizedglutathioneconcentrations.
| Catalog#7511-100-Kincludes: | ||
| CatalogNumber | Description | Qty |
| 7511-100-01 | GlutathioneReductase | 1 |
| 7511-100-02 | 25XAssayBuffer | 1 |
| 7511-100-04 | ReactionMix | 1 |
| 7511-100-05 | 96-WellPlates | 1 |
| 7511-100-06 | 4µMGSSG | 1 |
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