Description
CONCENTRATION
250μMindeionizedwater.
ExtinctionCoefficient
EmM(265nM)=22
1083.93g/mole(1060.94g/moleasafreeacid)
STORAGE
Storeat-80°C.
APPLICATIONS
ActivitymeasurementsofNAD+-requiringenzymes.
AssaystoidentifyinhibitorsofNAD+-requiringenzymes.
BacilluscereusCerthraxADP-ribosylatesVinculintoDisruptFocalAdhesionComplexesandCellAdhesion,
NathanC.SimonandJosephT.Barbieri
J.Biol.Chem.,Apr2014;289:10650–10659.
http://www.jbc.org/cgi/content/abstract/289/15/10650
Theoligonucleotide/oligosaccharide-bindingfoldmotifisapoly(ADP-ribose)-bindingdomainthatmediatesDNAdamageresponse
Zhang,F.,Chen,Y.,Li,M.andYu,X.
PNAS,May2014;111:7278–7283.
http://www.pnas.org/cgi/content/abstract/111/20/7278
BEC,aNovelEnterotoxinofClostridiumperfringensFoundinHumanClinicalIsolatesfromAcuteGastroenteritisOutbreaks
ShinyaYonogi,ShigeakiMatsuda,TakaoKawai,TomokoYoda,TetsuyaHarada,YukoKumeda,KazuyoshiGotoh,HirotakaHiyoshi,ShotaNakamura,ToshioKodama,andTetsuyaIida
Infect.Immun.,Jun2014;82:2390–2399.
http://iai.asm.org/cgi/content/abstract/82/6/2390
NAD-dependentADP-ribosylationofthehumanantimicrobialandimmune-modulatorypeptideLL-37byADP-ribosyltransferase-1
MonicaPicchianti,CarlaRusso,MartaCastagnini,MassimilianoBiagini,ELISAbettaSoldaini,andEnricoBalducci
InnateImmunity,Aug2014;10.1177/1753425914536242.
http://ini.sagepub.com/cgi/content/abstract/1753425914536242v1
NeuronaldeathinducedbymisfoldedprionproteinisduetoNAD+depletionandcanberelievedinvitroandinvivobyNAD+replenishment
MinghaiZhou,GregoryOttenberg,GianFrancoSferrazza,ChristopherHubbs,MohammadFallahi,GavinRumbaugh,AliciaF.Brantley,andCorinneI.Lasmézas
Brain,Feb2015;10.1093/brain/awv002.
http://brain.oxfordjournals.org/cgi/content/abstract/awv002v1
Tankyrase-mediatedβ-cateninactivityregulatesvasopressin-inducedAQP2expressioninkidneycollectingductmpkCCDc14cells
HyunJunJung,Sang-YeobKim,Hyo-JungChoi,Eui-JungPark,Jung-SukLim,JørgenFrøkiaer,SørenNielsen,andTae-HwanKwon
AmJPhysiolRenalPhysiol,Mar2015;308:F473–F486.
http://ajprenal.physiology.org/cgi/content/abstract/308/5/F473
StructuralBasisforLackofADP-ribosyltransferaseActivityinPoly(ADP-ribose)Polymerase-13/ZincFingerAntiviralProtein
TobiasKarlberg,MirjamKlepsch,Ann-GerdThorsell,C.DavidAndersson,AnnaLinusson,andHerwigSchüler
J.Biol.Chem.,Mar2015;290:7336–7344.
http://www.jbc.org/cgi/content/abstract/290/12/7336
“NeuronaldeathinducedbymisfoldedprionproteinisduetoNAD+depletionandcanberelievedinvitroandin
vivobyNAD+replenishment”
MinghaiZhou,GregoryOttenberg,GianFrancoSferrazza,ChristopherHubbs,MohammadFallahi,GavinRumbaugh,AliciaF.Brantley,andCorinneI.Lasmézas
Brain,Apr2015;138:992–1008.
http://brain.oxfordjournals.org/cgi/content/abstract/138/4/992
“ProteinKinaseInhibitorH89EnhancestheActivityofPseudomonasExotoxinA–BasedImmunotoxins”
XiufenLiu,FABIanMüller,AlanS.Wayne,andIraPastan
Mol.CancerTher.,May2016;15:1053–1062.
http://mct.aacrjournals.org/cgi/content/abstract/15/5/1053
“FluorescentsensorsofPARP-1structuraldynamicsandallostericregulationinresponsetoDNAdamage”
JaminD.Steffen,MichaelM.McCauley,andJohnM.Pascal
NucleicAcidsRes.,Aug2016;10.1093/nar/gkw710.
http://nar.oxfordjournals.org/cgi/content/abstract/gkw710v1
“TheNarEproteinofNeisseriagonorrhoeaecatalyzesADP-ribosylationofseveralADP-riboseacceptorsdespiteanN-terminaldeletion”
“PaulaI.Rodas,A.SaidÁlamos-Musre,FranciscaP.Álvarez,AlejandroEscobar,CeciliaV.Tapia,Eduardo
Osorio,CarolinaOtero,IvánL.Calderón,JuanA.Fuentes,FernandoGil,DanielParedes-Sabja,andMyronChristodoulides”
FEMSMicrobiolLett,Aug2016;363:fnw181
http://femsle.oxfordjournals.org/cgi/content/abstract/363/17/fnw181
ThePARPInhibitorAZD2461ProvidesInsightsintotheRoleofPARP3InhibitionforBothSyntheticLethalityandTolerabilitywithChemotherapyinPreclinicalModels
“LenkaOplustilO’Connor,StuartL.Rulten,AaronN.Cranston,RajeshOdedra,HenryBrown,JannekeE.
Jaspers,LouiseJones,CharlotteKnights,BastiaanEvers,AttillaTing,RobertH.Bradbury,MarinaPajic,
SvenRottenberg,JosJonkers,DavidRudge,NiallM.B.Martin,KeithW.Caldecott,AlanLau,andMarkJ.
O’Connor”
ThePARPinhibitorAZD2461wasdevelopedasanext-generationagentfollowingolaparib,thefirstPARPinhibitorapprovedforcancertherapy.InBRCA1-deficientmousemodels,olaparibresistancepredominantlyinvolvesoverexpressionofP-glycoprotein,soAZD2461wasdevelopedasapoorsubstratefordrugtransporters.Herewedemonstratetheefficacyofthiscompoundagainstolaparib-resistanttumorsthatoverexpressP-glycoprotein.Inaddition,AZD2461wasbettertoleratedincombinationwithchemotherapythanolaparibinmice,whichsuggeststhatAZD2461couldhavesignificantadvantagesoverolaparibintheclinic.However,thissuperiortoxicityprofiledidnotextendtorats.InvestigationsofthisdifferencerevealedadifferentialPARP3inhibitoryactivityforeachcompoundandahigherlevelofPARP3expressioninbonemarrowcellsfrommiceascomparedwithratsandhumans.OurfindingshaveimplicationsfortheuseofmousemodelstoassessbonemarrowtoxicityforDNA-damagingagentsandinhibitorsoftheDNAdamageresponse.Finally,structuralmodelingofthePARP3-activesitewithdifferentPARPinhibitorsalsohighlightsthepotentialtodevelopcompoundswithdifferentPARPfamilymemberspecificityprofilesforoptimalantitumoractivityandtolerability.CancerRes;76(20);6084-94.(C)2016AACR.
http://cancerres.aacrjournals.org/cgi/content/abstract/76/20/6084
WideVariabilityintheTimeRequiredforImmunotoxinstoKillBLineageAcuteLymphoblasticLeukemiaCells:ImplicationsforTrialDesign
FabianMüller,TylerCunningham,XiuFenLiu,AlanS.Wayne,andIraPastan
Clin.CancerRes.,Oct2016;22:4913–4922
http://clincancerres.aacrjournals.org/cgi/content/abstract/22/19/4913
FluorescentsensorsofPARP-1structuraldynamicsandallostericregulationinresponsetoDNAdamage
JaminD.Steffen,MichaelM.McCauley,andJohnM.Pascal
NucleicAcidsRes.,Nov2016;44:9771–9783
http://nar.oxfordjournals.org/cgi/content/abstract/44/20/9771
