Description
CONCENTRATION
250μMindeionizedwater.
Thereisa1:1stoichiometryforincorporation;oneFluoresceinlabelforeachNAD+molecule.
ExtinctionCoefficient
EmM(262nM)=38
STORAGE
Storeat-80°C.
APPLICATIONS
ActivitymeasurementsofNAD+-requiringenzymes.
AssaystoidentifyinhibitorsofNAD+-requiringenzymes.
C3larvinToxin,anADP-ribosyltransferasefromPaenibacilluslarvae
DanielKrska,RavikiranRavulapalli,RobertJ.Fieldhouse,MiguelR.Lugo,andA.RodMerrill
J.Biol.Chem.,Jan2015;290:1639–1653
http://www.jbc.org/cgi/content/abstract/290/3/1639
AQuantitativeAssayRevealsLigandSpecificityoftheDNAScaffoldRepairProteinXRCC1andEfficientDisassemblyofComplexesofXRCC1andthePoly(ADP-ribose)Polymerase1byPoly(ADP-ribose)Glycohydrolase
In-KwonKim,RoderickA.Stegeman,ChrisA.Brosey,andTomEllenberger
J.Biol.Chem.,Feb2015;290:3775–3783.
http://www.jbc.org/cgi/content/abstract/290/6/3775