Description
Duringtheprocessofapoptosis,DNAfragmentationoccursfollowingtheactivationofendonucleases.Thelabelingofthe3’endsofDNAfragmentsprovidesaneasymeasureofcellsundergoingapoptosis.Modifiednucleotidesareincorporatedatthe3’endsbytheactivityofterminaldeoxynucleotidyltransferase(TdT).ThesenucleotidesaredetectedusingahorserADIsh-peroxidasedetectionsystemandTACS-Sapphire™.TiterTACS™canbeusedwithsUSPensionormonolayercells.Thekitisdesignedtousefixedsamples,allowingyoutoworksafelywithsamplesthatareinfectedwithbiohazardousagents.Fixedsamplesmaybestoredconvenientlyduringtime-courseexperiments.TheTiterTACS™KitalsoprovidesTACS-Nuclease™solutiontogeneratepositivecontrolsfromyourownsamplesgivingyouamaximalvaluefortheassay.
DetectionusingTACS-Sapphire™,anon-toxiccolorimetricsubstrate,allowsbothkineticandendpointreadings.Thereactiongeneratesablueproductthatcanbemeasuredat630nm,andwillturnyellowafterthereactionisstoppedwithacidallowingendpointreadingat450nm.
FEATURES:
- Convenient,96wellmicroplateformat.
- Quantitativecolorimetricdetection.
- IncludesbothProteinaseKandexclusiveCytonin™non-enzymaticpermeABIlizationreagents.
- IncludesTACS-Nucleasesolutionforpreparingsample-dependentpositivecontrols.
APPLICATIONS:
Identificationandquantitationofapoptosisinculturedcellsina96wellassay.
| Catalog#4822-96-Kincludes: | ||
| CatalogNumber | Description | Qty |
| 4800-30-01 | ProteinaseK | 2 |
| 4800-30-06 | Streptavidin-HRP | 1 |
| 4800-30-12 | BlueStrep-HRPDiluent | 1 |
| 4800-30-15 | TACS-Nuclease™ | 1 |
| 4800-30-16 | TACS-Nuclease™Buffer | 1 |
| 4810-30-04 | TACS2TdTdNTP | 1 |
| 4810-30-14 | 50xManganeseCation | 2 |
| 4817-60-02 | 10XTdTLabelingBuffer | 1 |
| 4817-60-03 | 10XTdTStopBuffer | 1 |
| 4821-96-05 | TdTEnzyme | 1 |
| 4822-96-08 | TACS-Sapphire™ | 1 |
| 4876-05-01 | Cytonin™ | 1 |
“ADAM12:ageneticmodifierofpreclinicalperipheralarterialdisease”
“AyotundeO.Dokun,LingdanChen,MitsuharuOkutsu,CharlesR.Farber,SuroviHazarika,W.Schuyler
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AmJPhysiolHeartCircPhysiol,Sep2015;309:H790–H803.
http://ajpheart.physiology.org/cgi/content/abstract/309/5/H790
“DesensitizationofidiopathicpulmonaryfibrosisfibroblaststoAlternariaalternataextract-mediatednecroticcelldeath”
JintaekIm,KyutaeKim,JiYoungYhee,ScottM.O’Grady,andRichardS.NhoPHY2,Nov2016;4:e13020.
http://physreports.physiology.org/cgi/content/abstract/4/21/e13020
