Description
TheextracellularscaffoldinginskintissuecanmakeitproblematictopermeABIlizeandhighbackgroundisacommonproblemwithskinsamples.TheDermaTACS™Kitincludestwopermeabilizationreagentsasoptionsforpermeabilizationalongwithadetailedprotocolwithhintsandtipsforoptimallabelingofskinsamplesbasedonempiricaltesting.LowbackgroundisachievedusingacombinationofthehighspecificityofTrevigen’suniquecation-basedoptimizedTdTlabelingreactionusingApoptosisGrade™TdTandTACSBlueLabel™asthevisualizationmethod.AlsoincludedisTACS-Nuclease™togenerateapositivecontrolfromyourownsample.InconjunctionwiththeexcellentcontrastbetweentheTACSBlueLabel™andtheRedCounterstainC,thepositivecontrolensureseasydatainterpretation.
Thiscompletekitprovidesallthereagentsrequiredforlabelingincludingtwopermeabilizationreagents,labelinganddetectionreagents,stopbuffer,counterstain,andTACS-Nuclease™.
| CatalogNumber | Description | Qty |
| 4800-30-01 | ProteinaseK | 1 |
| 4800-30-06 | Streptavidin-HRP | 1 |
| 4800-30-11 | TACS-BlueLabel™ | 1 |
| 4800-30-15 | TACS-Nuclease™ | 1 |
| 4800-30-16 | TACS-Nuclease™Buffer | 1 |
| 4800-30-19 | RedCounterstainC | 1 |
| 4810-30-02 | TACS®2TdTLabelingBuffer | 1 |
| 4810-30-03 | TACS®2TdTStopBuffer | 1 |
| 4810-30-05 | TdTEnzyme | 1 |
| 4828-30-04 | B-dNTPMix | 1 |
| 4828-30-06 | anti-BrdUantibody | 1 |
| 4828-30-12 | Strep-Diluent | 1 |
| 4876-05-01 | Cytonin™ | 1 |
TargeteddeletionofRasGRP1impairsskintumOrigenesis
Sharma,A.,Fonseca,L.L.,Rajani,C.,Yanagida,J.K.,Endo,Y.,Cline,J.M.,Stone,J.C.,Ji,J.,Ramos,J.W.,andLorenzo,P.S.
Carcinogenesis,Feb2014;10.1093/carcin/bgu016
http://carcin.oxfordjournals.org/cgi/content/abstract/bgu016v2
