Description
Thelabelingoftumor-containingspecimenscanbeproblematicduetothehighlevelsofnecrosisinthesetissues.Duringnecrosis,cessationofhomeostasis,cellswelling,andnuclearmembranerupturegenerateshighlydegradedDNAthatcaninterferewithinterpretationofdata.
Trevigen’skithasanoptimizedTUNEL-basedsystemthatpreferentiallylabelsthedouble-strandedDNAfoundinapoptoticcells.DNAfragmentsgeneratedduringapoptosisareend-labeledwithmodifiednucleotidesusingahighlypurifiedterminaldeoxynucleotidyltransferaseenzyme(TdT)inauniquenon-toxiclabelingbuffersupplementedwithatailoredcationtomaximizethe signal-to-noiseratio.TheincorporatednucleotidesaresubsequentlydetectedusingahorserADIshperoxidaseconjugate.Theconjugatecatalyzestheconversionofdiaminobenzidine(DAB)intoavisIBLedarkbrownprecipitate.ThekitincludesmethylgreencounterstainwhichisageneralcounterstainappropriateforawidevarietyoftissuesandtumortypesandprovidesgoodcontrastwiththebrownDABinlabeledcells.ADABenhancerisalsoprovidedwiththekitfortheoptiontointensifyordarkenDABstaining.
TumorTACS™containsallthereagentsrequiredforpermeABIlizing,labeling,staining,andcounterstainingtumorsamples,includingTACS-Nuclease™forpreparingpositivecontrols.
FEATURES:
- Uniquebuffersystemproducesmoreconsistentlabeling.
- Performancetestedontumorsamples.
- IncludesbothProteinaseKand exclusivenon-enzymaticCytonin™permeabilizationreagent.
- IncludesTACS-Nuclease™solutionforpreparingsample-dependentpositivecontrols.
- Helpsresolveuniqueproblemsencounteredwhenusingtissuesorcellsfromtumors.
APPLICATIONS:
- Insitudetectionofapoptosisinfixedfrozen,paraffinembedded,orplasticembeddedcellsandtissues.
- Assistsintheidentificationofapoptoticmorphologies.
| Catalog#4815-30-Kincludes: | ||
| CatalogNumber | Description | Qty |
| 4800-30-01 | ProteinaseK | 1 |
| 4800-30-06 | Streptavidin-HRP | 1 |
| 4800-30-07 | Diaminobenzidine | 1 |
| 4800-30-09 | DABEnhancer | 1 |
| 4800-30-15 | TACS-Nuclease™ | 1 |
| 4800-30-16 | TACS-Nuclease™Buffer | 1 |
| 4800-30-18 | MethylGreen1% | 1 |
| 4810-30-02 | TACS®2TdTLabelingBuffer | 1 |
| 4810-30-03 | TACS®2TdTStopBuffer | 1 |
| 4810-30-04 | TACS®2TdTdNTP | 1 |
| 4810-30-05 | TdTEnzyme | 1 |
| 4810-30-14 | 50xManganeseCation | 1 |
| 4876-05-01 | Cytonin™ | 1 |
“AnticancerdrugcandidateCBL0137,whichinhibitshistonechaperoneFACT,isefficaciousinpreclinicalorthotopicmodelsoftemozolomideresponsiveand-resistantglioblastoma”
“TaraA.Barone,CatherineA.Burkhart,AlfiyaSafina,GaryHaderski,KaterinaV.Gurova,AndreiA.Purmal,AndreiV.Gudkov,andRobertJ.Plunkett”
NeuroOncology,Jul2016;10.1093/neuonc/now141.
http://neuro-oncology.oxfordjournals.org/cgi/content/abstract/now141v1
