Description

EnzymaticincorporationofbiotinylatednucleotidesinfragmentedDNAisperformedbyahighlypurifiedformoftheTdTenzymeinanon-toxiclabelingbuffer.Biotinlabelingisdetectedusingafluoresceinconjugateofstreptavidinandvisualizedbyepifluorescencemicroscopy.ThiscompletekitprovidesallthereagentsrequiredforlabelingincludingtwopermeABIlizationreagents,labelinganddetectionreagents,stopbuffers,counterstain,andTACS-Nuclease™reagentsforgeneratingpositivecontrolswithyourownsamples.Pleaserefertoourcomponents listingfordetails.

FEATURES:

• TdTbasedinsitulabeling.
• Fluorescencedetection.
• Cationoptimizationoptionforhighsignalandlowbackground.
• IncludesbothProteinaseKandexclusiveCytonin™ non-enzymaticpermeabilizationreagent.
• IncludesTACS-Nuclease™controlreagents.

APPLICATIONS:

• Insitudetectionofapoptosisinfixedfrozen,paraffinembedded,orplasticembeddedcellsandtissues.
• Fluorescencemicroscopy.

CucurbitacinIInducesProtectiveAutophagyinGlioblastomainVitroandinVivo
GuangYuan,Shao-FengYan,HaoXue,PingZhang,Jin-TangSun,andGangLi
J.Biol.Chem.,Apr2014;289:10607–10619
http://www.jbc.org/cgi/content/abstract/289/15/10607

ThePARPInhibitors,VeliparibandOlaparib,AreEffectiveChemopreventiveAgentsforDelayingMammaryTumorDevelopmentinBRCA1-deficientMice
CiricTo,Eun-HeeKim,DarleneB.Royce,CharlotteR.Williams,RyanM.Collins,ReneeRisingsong,MichaelB.Sporn,andKarenT.Liby
CancerPreventionResearch,Jul2014;7:698–707
http://cancerpreventionresearch.aacrjournals.org/cgi/content/abstract/7/7/698

InhibitionofIntracellularClusterinAttenuatesCellDeathinNephropathicCystinosis
PoonamSansanwal,LiLi,andMinnieM.Sarwal
J.Am.Soc.Nephrol.,Jul2014;10.1681/ASN.2013060577.
http://jasn.asnjournals.org/cgi/content/abstract/ASN.2013060577v1

Thymosin-β4preventscardiacruptureandimprovescardiacfunctioninmicewithmyocardialinfarction
HongmeiPeng,JiangXu,Xiao-PingYang,XiangguoDai,EdwardL.Peterson,OscarA.Carretero,andNour-EddineRhaleb
AmJPhysiolHeartCircPhysiol,Sep2014;307:H741–H751
http://ajpheart.physiology.org/cgi/content/abstract/307/5/H741

Secretedfibroblast-derivedmiR-34ainducestubularcellapoptosisinfibrotickidney
YangZhou,MingxiaXiong,JingNiu,QiSun,WeifangSu,KeZen,ChunsunDai,andJunweiYang
J.CellSci.,Oct2014;127:4494–4506.
http://jcs.BIOLOGists.org/cgi/content/abstract/127/20/4494

HumanN-methylD-aspartatereceptorantibodiesaltermemoryandbehaviourinmice
JesúsPlanagumà,FrankLeypoldt,FrancescoMannara,JavierGutiérrez-Cuesta,ElenaMartín-García,EstherAguilar,MaartenJ.Titulaer,MarPetit-Pedrol,AnkitJain,RitaBalice-Gordon,MelikeLakadamyali,FrancescGraus,RafaelMaldonado,andJosepDalmau
Brain,Nov2014;10.1093/brain/awu310.
http://brain.oxfordjournals.org/cgi/content/abstract/awu310v1

Brain-DerivedNeurotrophicFactorDeficiencyRestrictsProliferationofOligodendrocyteProgenitorsFollowingCuprizone-InducedDemyelination
VlADIslavTsiperson,YangyangHuang,IssaBagayogo,YeriSong,MelissaWVonDran,EmanuelDiCicco-Bloom,andCherylFDreyfus
ASNNeuro,Jan2015;7:1759091414566878.
http://asn.sagepub.com/cgi/content/abstract/7/1/1759091414566878

“AlteredPhenotypeofβ-CellsandOtherPancreaticCellLineagesin
PatientsWithDiffuseCongenitalHyperinsulinisminInfancyCausedbyMutationsintheATP-SensitiveK-Channel”
“RachelJ.Salisbury,BingHan,RachelE.Jennings,AndrewA.Berry,AdamStevens,ZainabMohamed,
SarahA.Sugden,RonaldDeKrijger,SarahE.Cross,PaulP.V.Johnson,MelanieNewbould,KarenE.
Cosgrove,KarenPiperHanley,IndraneelBanerjee,MarkJ.Dunne,andNeilA.Hanley”
Diabetes,Sep2015;64:3182–3188.
http://diabetes.diabetesjournals.org/cgi/content/abstract/64/9/3182

“DHAsuppresseschronicapoptosisinthelungcausedbyperinatal
inflammation”
MehboobAli,KathrynM.Heyob,MarkusVelten,TrentE.Tipple,andLynetteK.Rogers
AmJPhysiolLungCellMolPhysiol,Sep2015;309:L441–L448
http://ajplung.physiology.org/cgi/content/abstract/309/5/L441

HomeostaticresponsesofcolonicLGR5+stemcellsfollowingacuteinvivoexposuretoagenotoxiccarcinogen
EunjooKim,LaurieA.Davidson,RogerS.Zoh,MarthaE.Hensel,BhimanagoudaS.Patil,GuddadarangavvanahallyK.Jayaprakasha,EvelynS.Callaway,ClintonD.Allred,NancyD.Turner,BradR.Weeks,andRobertS.Chapkin
Carcinogenesis,Feb2016;37:206–214
http://carcin.oxfordjournals.org/cgi/content/abstract/37/2/206

“DichloroacetatePreventsCisplatin-InducedNephrotoxicitywithoutCompromisingCisplatinAnticancerProperties”
RamindhuGalgamuwa,KristineHardy,JaneE.Dahlstrom,AnnekeC.Blackburn,ElizeWium,MelissaRooke,JeanY.Cappello,PadmajaTummala,HardipR.Patel,AaronChuah,LuyangTian,LindaMcMorrow,PhilipG.Board,andAngeloTheodoratos
J.Am.Soc.Nephrol.,Mar2016;10.1681/ASN.2015070827
http://jasn.asnjournals.org/cgi/content/abstract/ASN.2015070827v1

“DichloroacetatePreventsCisplatin-InducedNephrotoxicitywithoutCompromisingCisplatinAnticancerProperties”
“RamindhuGalgamuwa,KristineHardy,JaneE.Dahlstrom,AnnekeC.Blackburn,ElizeWium,Melissa
Rooke,JeanY.Cappello,PadmajaTummala,HardipR.Patel,AaronChuah,LuyangTian,LindaMcMorrow,PhilipG.Board,andAngeloTheodoratos”
J.Am.Soc.Nephrol.,Nov2016;27:3331–3344.
http://jasn.asnjournals.org/cgi/content/abstract/27/11/3331