Description

ThiscompletekitprovidesallthereagentsrequiredforlabelingincludingtwopermeABIlizationreagents,labelinganddetectionreagents,stopbuffers,counterstain,andTACS-Nuclease™reagentsforgeneratingpositivecontrolswithyourownsamples.Pleaserefertoourcomponents listingfordetails.

FEATURES:

• TdTbasedinsitulabeling.
• Cationoptimizationforhighsignalandlowbackground.
• CompletekitprovidingDABandMethylGreenCounterstain.
• IncludesbothProteinaseKandexclusiveCytonin™non-enzymaticpermeabilizationreagent.
• IncludesTACS-Nuclease™controlreagents.

APPLICATIONS:

• InsitudetectionofapoptosisbyTUNEL-basedassayinfixedfrozen,paraffinembedded,orplasticembeddedcellsandtissues.
• Lightmicroscopy.

Tumorpromoter-inducedsulfiredoxinisrequiredformouseskintumOrigenesis
Wu,L.,Jiang,H.,Chawsheen,H.A.,Mishra,M.,Young,M.R.,Gerard,M.,Toledano,M.B.,Colburn,N.H.,andWei,Q.Tumorpromoter-inducedsulfiredoxinisrequiredformouseskintumorigenesis41696CarcinogenesisCarcinogenesis
Carcinogenesis,Feb2014;10.1093/carcin/bgu035
http://carcin.oxfordjournals.org/cgi/content/abstract/bgu035v2

ReductionofNa/K-ATPaseaffectscardiacremodelingandincreasesc-kitcellabundanceinpartialnephrectomizedmice
ChristopherA.Drummond,MoustafaSayed,KaleighL.Evans,HuilinShi,XiaoliangWang,StevenT.Haller,JiangLiu,ChristopherJ.Cooper,ZijianXie,JosephI.Shapiro,andJiangTian
AmJPhysiolHeartCircPhysiol,Jun2014;306:H1631–H1643.
http://ajpheart.physiology.org/cgi/content/abstract/306/12/H1631

RoleofLeukotrieneA4HydrolaseAminopeptidaseinthePathogenesisofEmphysema
MikellPaige,KanWang,MarieBurdick,SunhyePark,JosiahCha,ErinJeffery,NicholasSherman,andY.MichaelShim
J.Immunol.,Jun2014;192:5059–5068.
http://www.jimmunol.org/cgi/content/abstract/192/11/5059

Intestinalmyofibroblast-specificTpl2-Cox-2-PGE2pathwaylinksinnatesensingtoepithelialhomeostasis
ManolisRoulis,ChristoforosNikolaou,ElenaKotsaki,EleannaKaffe,NikiKaragianni,VasilikiKoliaraki,KleliaSalpea,JiannisRagoussis,VassilisAidinis,EvaMartini,ChristophBecker,HarveyR.Herschman,StefaniaVetrano,SilvioDanese,andGeorgeKollias
PNAS,Oct2014;111:E4658–E4667.
http://www.pnas.org/cgi/content/abstract/111/43/E4658

SyntheticlethalityofcombinedglutaminaseandHsp90inhibitioninmTORC1-driventumorcells
JingLi,AlfredoCsibi,SunYang,GregoryR.Hoffman,ChenggangLi,ErikZhang,JaneJ.Yu,andJohnBlenis
PNAS,Jan2015;112:E21–E29
http://www.pnas.org/cgi/content/abstract/112/1/E21

“NeonatalUterineandVaginalCellProliferationandAdenogenesisAreIndependentofEstrogenReceptor1(ESR1)intheMouse”
ManjunathaK.Nanjappa,TheresaI.Medrano,AmeliaG.March,andPaulS.Cooke
BiolReprod,Mar2015;92:78.
http://www.biolreprod.org/cgi/content/abstract/92/3/78

TheEndogenousCell-FateFactorDachshundRestrainsProstateEpithelialCellMigrationviaRepressionofCytokineSecretionviaaCXCLSignalingModule
“KeChen,KongmingWu,XuanmaoJiao,LipingWang,XiaomingJu,MinWang,
GabrieleDiSante,ShaohuaXu,QiongWang,KevinLi,XinSun,CongwenXu,Zhiping
Li,MathewC.Casimiro,AdamErtel,SankarAddya,PeterA.McCue,MichaelP.Lisanti,ChenguangWang,RichardJ.Davis,GraemeMardon,andRichardG.Pestell”
CancerRes.,May2015;75:1992–2004.
http://cancerres.aacrjournals.org/cgi/content/abstract/75/10/1992

“DistinctImmuneResponsesinResistantandSusceptIBLeStrainsof
MiceduringNeurovirulentAlphavirusEncephalomyelitis”
KirstenA.Kulcsar,VictoriaK.Baxter,RachyAbraham,AshleyNelson,andDianeE.Griffin
J.Virol.,Aug2015;89:8280–8291.
http://jvi.asm.org/cgi/content/abstract/89/16/8280

SecretedFrizzled-RelatedProtein3(SFRP3)IsRequiredforTumorigenesisofPAX3–FOXO1-PositiveAlveolarRhaBDomyosarcoma
JulieJ.G.Kephart,RosanneG.J.Tiller,LisaE.S.Crose,KatherineK.Slemmons,Po-HanChen,AshleyR.Hinson,RexC.Bentley,Jen-TsanAshleyChi,andCorinneM.Linardic
Clin.CancerRes.,Nov2015;21:4868–4880.
http://clincancerres.aacrjournals.org/cgi/content/abstract/21/21/4868
Accelerationofdiabeticwoundhealingusinganovelprotease–anti-proteasecombinationtherapy
MingGao,TrungT.Nguyen,MarkA.Suckow,WilliamR.Wolter,MajorGooyit,ShahriarMobashery,andMaylandChang
PNAS,Dec2015;112:15226–15231.
http://www.pnas.org/cgi/content/abstract/112/49/15226

TargetedDeletionofp53inLgr5-ExpressingIntestinalStemCellsPromotesColonTumorigenesisinaPreclinicalModelofColitis-AssociatedCancer
LaurieA.Davidson,EvelynS.Callaway,EunjooKim,BradR.Weeks,Yang-YiFan,ClintonD.Allred,andRobertS.Chapkin
CancerRes.,Dec2015;75:5392–5397.
http://cancerres.aacrjournals.org/cgi/content/abstract/75/24/5392

FolateReceptor–TargetedPolymericMicellarNanocarriersforDeliveryofOrlistatasaRepurposedDrugagainstTriple-NegativeBreastCancer
RamasamyPaulmurugan,RohithBhethanabotla,KaushikMishra,RammohanDevulapally,KiraFoygel,ThillaiV.Sekar,JeyaramaS.Ananta,TarikF.Massoud,andAbrahamJoy
Mol.CancerTher.,Feb2016;15:221–231
http://mct.aacrjournals.org/cgi/content/abstract/15/2/221

Transientincreaseoffractionalanisotropyinreversiblevasogenicedema
ShihokoKimura-Ohba,YiYang,JeffreyThompson,TomonoriKimura,VictorMSalayandia,MelissaCosse,YirongYang,LaurelOSillerud,andGaryARosenberg
JournalofCerebralBloodFlowandMetabolism,Feb2016;10.1177/0271678X16630556.
http://jcb.sagepub.com/cgi/content/abstract/0271678X16630556v1

“TLR4DeficiencyImpairsOligodendrocyteFormationintheInjuredSpinalCord”
“JamieS.Church,KristinaA.Kigerl,JessicaK.Lerch,PhillipG.Popovich,andDanaM.McTigue”
J.Neurosci.,Jun2016;36:6352–6364
http://www.jneurosci.org/cgi/content/abstract/36/23/6352

“mTORC1-DrivenTumorCellsAreHighlySensitivetoTherapeutic
TargetingbyAntagoNISTsofOxidativeStress”
“JingLi,SejeongShin,YangSun,Sang-OhYoon,ChenggangLi,ErikZhang,JaneYu,JianmingZhang,andJohnBlenis”
CancerRes.,Aug2016;76:4816–4827.
http://cancerres.aacrjournals.org/cgi/content/abstract/76/16/4816

“Smpd3ExpressioninbothChondrocytesandOsteoblastsIsRequired
forNormalEndochondralBoneDevelopment”
“JingjingLi,GarthigaManickam,SeemunRay,Chun-doOh,HideyoYasuda,PierreMoffatt,andMonzurMurshed”
Mol.Cell.Biol.,Sep2016;36:2282–2299
http://mcb.asm.org/cgi/content/abstract/36/17/2282

Uterinenaturalkillercellpartnershipsinearlymousedeciduabasalis
AllisonM.FelkerandB.AnneCroy
J.Leukoc.Biol.,Oct2016;100:645–655
http://www.jleukbio.org/cgi/content/abstract/100/4/645