Description
FlowTACS™alsoprovidesflexibilityinselectionoffluorophoresthatarecompatIBLewithyourresearchdesign.Thekitallowsmulti-colorlabelinginconjunctionwithexperiment-specificantibodies.
DNAfragmentationisanirreversible,committedstepinapoptosis,andthelabelingof3’endsprovidesaneasymeasureofcellsundergoingapoptosis.CellsmayalsobeanalyzedforDNAcontentusingtheincludedpropidiumiodide/RNaseAsolution.TheFlowTACS™KitalsoprovidesTACS-Nuclease™togeneratepositivecontrolsforcalibratingyourflowcytometer.TheFlowTACS™Kitusesfixedcells,allowingyoutostoresamplesduringtime-courseexperimentandtosafelyworkwithbiohazardousagents.
ThiscompletekitprovidesallthereagentsrequiredforlabelingincludingtwopermeABIlizationreagents,labelingandstopbuffers,labelinganddetectionreagents,andTACS-Nuclease™forgeneratingpositivecontrolswithyourownsamples.Pleaserefertoourcomponentslistingfordetails.
FEATURES:
- Designedforusewithfixedcells.
- Includesexclusivenon-enzymaticCytonin™permeabilizationreagent.
- IncludesTACS-Nuclease™solutionforpreparingsample-dependentpositivecontrols.
APPLICATIONS:
- IdentificationandquantitationoffixedapoptoticcellsinsUSPensionbyFlowCytometry.
- Allowsbi-colorandtri-coloranalysis.
| Catalog#4817-60-Kincludes: | ||
| CatalogNumber | Description | Qty |
| 4800-30-14 | Streptavidin-FITC | 1 |
| 4800-30-15 | TACS-Nuclease™ | 1 |
| 4800-30-16 | TACS-Nuclease™Buffer | 1 |
| 4810-30-04 | TACS®2TdTdNTP | 1 |
| 4810-30-05 | TdTEnzyme | 1 |
| 4810-30-14 | 50xManganeseCation | 1 |
| 4817-60-02 | 10XTdTLabelingBuffer | 1 |
| 4817-60-03 | 10XTdTStopBuffer | 1 |
| 4817-60-04 | PI/RNase | 1 |
| 4876-05-01 | Cytonin™ | 1 |
